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KMID : 0605920160220020023
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Journal of Korean Association of Pediatric Surgeons
2016 Volume.22 No. 2 p.23 ~ p.28
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The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease
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Jeong Jae-Yeop
Kim Sang-Pyo
Jung Eun-Young
Choi Soon-Ok
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Abstract
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Purpose: Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE.
Methods: Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining.
Results: Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%.
Conclusion: We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
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KEYWORD
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Hirschsprung disease, Calbindin 2, Acetylcholinesterase, Hematoxylin, Diagnosis
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